Nucleic acid and antigen detection tests for leptospirosis

nucleic acid testing for the detection of hbv dna

nucleic acid-based approaches for detection of viral hepatitis

conclusions despite rapidity, automation, accuracy, cost-effectiveness, high sensitivity, and high specificity of molecular techniques, each type of molecular technology has its own advantages and disadvantages. results various types of molecular biology tools can be used to detect and quantify viral genomic elements and analyze the sequences. these molecular assays are proper technologies for ...

Gel purified LipL32: a prospective antigen for detection of leptospirosis.

BACKGROUND Leptospirosis, a zoonosis, is a re-emerging disease, affecting populations across the globe. However, the current methods of diagnosis are time- consuming, cumbersome, imprecise or expensive. AIM To develop an assay for differential and early diagnosis of Leptospirosis. METHODS AND MATERIAL IgG based ELISA for evaluation of three antigens, namely, a gel-purified recombinant prote...

A handheld magnetic sensing platform for antigen and nucleic acid detection.

The core requirements for point-of-care (POC) diagnostics necessitate low-cost, portability, easily integrated sample preparation, and quick measurement time. Frequency-shift based magnetic sensing is a measurement technique utilizing a complementary metal-oxide-semiconductor (CMOS) integrated-circuit (IC) chip for magnetic label detection. The sensing scheme leverages the low-cost manufacturin...

Fluorescent hybridization probes for nucleic acid detection.

Due to their high sensitivity and selectivity, minimum interference with living biological systems, and ease of design and synthesis, fluorescent hybridization probes have been widely used to detect nucleic acids both in vivo and in vitro. Molecular beacons (MBs) and binary probes (BPs) are two very important hybridization probes that are designed based on well-established photophysical princip...